| Служба поликлональных антител |
| Номер пакета |
Описание |
Содержимое упаковки |
Time |
| Поликлональный пакет 1
(От белка к антисыворотке) |
l Customer provides antigen protein
l Protein will be checked by SDS-PAGE
l Immunize two New Zealand rabbits
l Titer the immunization by ELISA
l Post-bleed collection |
l 1-2ml pre-bleed
l 40-60ml post-bleed
l Project datasheet including ELISA data |
10-12 недели |
| Поликлональный пакет 2 (From protein to antigen purified antibody) |
l Customer provides antigen protein
l Protein will be checked by SDS-PAGE
l Immunize two New Zealand rabbits
l Titer the immunization by ELISA
l Post-bleed collection
l Antigen affinity purification
l Dot blot confirmation |
l 1-2ml pre-bleed
l 2-5mg affinity purified antibody
l Project datasheet including ELISA and dot blot data |
10-12 недели |
| Поликлональный пакет 3 (From peptide design to antiserum) |
l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)
l Synthesize one peptide (12-22aa, чистота>85%)
l Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l Immunize two New Zealand rabbits
l Titer the immunization by ELISA
l Post-bleed collection |
l 2-3mg antigen peptide
l 1-2ml pre-bleed
l 40-60ml post-bleed
l Project datasheet including ELISA data |
12-15 недели |
| Поликлональный пакет 4 (From peptide design to antigen purified antibodies) |
l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)
l Synthesize one peptide (12-22aa, чистота>85%)
l Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l Immunize two New Zealand rabbits
l Titer the immunization by ELISA
l Post-bleed collection
l Antigen affinity purification
l Dot blot confirmation |
l 2-3mg antigen peptide
l 1-2ml pre-bleed
l 2-5mg affinity purified antibody
l Project datasheet including ELISA and dot blot data |
12-15 недели |
| Поликлональный пакет 5 (От плазмиды к антисыворотке) |
l Клиент предоставляет вектор экспрессии
l Экспрессируйте и очищайте антигенный белок
l Immunize two New Zealand rabbits
l Titer the immunization by ELISA
l Post-bleed collection |
л 1 мг антигенного белка
l 1-2ml pre-bleed
l 40-60ml post-bleed
l Project datasheet including ELISA data |
12-15 недели |
| Поликлональный пакет 6 (От плазмиды к очищенному от антигена антителу) |
l Клиент предоставляет вектор экспрессии
l Экспрессируйте и очищайте антигенный белок
l Immunize two New Zealand rabbits
l Titer the immunization by ELISA
l Post-bleed collection
l Antigen affinity purification
l Dot blot confirmation |
л 1 мг антигенного белка
l 1-2ml pre-bleed
l 2-5mg affinity purified antibody
l Project datasheet including ELISA and dot blot data |
12-15 недели |
| Поликлональный пакет 7 (От кДНК к антисыворотке) |
l Клиент предоставляет информацию о белке
l Мы клонируем ген и создаем вектор экспрессии для гибридного белка.
l Экспрессируйте и очищайте антигенный белок
l Immunize two New Zealand rabbits
l Titer the immunization by ELISA
l Post-bleed collection |
l Рекомбинантный вектор
л 1 мг белка
l 1-2ml pre-bleed
l 40-60ml post-bleed
l Project datasheet including ELISA data |
14-16 недели |
| Поликлональный пакет 8 (От кДНК к очищенному от антигена антителу) |
l Клиент предоставляет информацию о генах и белках
l Мы клонируем ген и создаем вектор экспрессии для гибридного белка.
l Экспрессируйте и очищайте антигенный белок
l Immunize two New Zealand rabbits
l Titer the immunization by ELISA
l Post-bleed collection
l Antigen affinity purification
l Dot blot confirmation |
l Рекомбинантный вектор
л 1 мг белка
l 1-2ml pre-bleed
l 2-5mg affinity purified antibody
l Project datasheet including ELISA and dot blot data |
14-16 недели |
| Поликлональный пакет 9 (Фосфоспецифическое поликлональное антитело) |
l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)
l Synthesize one peptide (12-22aa, чистота>85%)
l Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l Immunize two New Zealand rabbits
l Titer the immunization by ELISA
l Post-bleed collection
l Antigen affinity purification |
л 2-3 мг пептидов/EA (нет- & люминофор- пептид)
l 1-2ml pre-bleed
l 2-5 мг антитела против нефосфопептида
l 2-5 мг фосфоспецифического антитела
l Project datasheet including ELISA and dot blot data |
13-16 недели |
| Поликлональный пакет 10 (Lys-монометилированное специфическое поликлональное антитело) |
l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)
l Synthesize one peptide (12-22aa, чистота>85%)
l Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l Immunize two New Zealand rabbits
l Titer the immunization by ELISA
l Post-bleed collection
l Antigen affinity purification |
л 2-3 мг пептидов/EA (нет- & монометилированный пептид)
l 1-2ml pre-bleed
l 2-5 мг антитела против неметилированного пептида
л 2-5 мг Лиза(Мне) специфическое антитело
l Project datasheet including ELISA and dot blot data |
13-16 недели |
| Поликлональный пакет 11 (Lys-диметилированное специфическое поликлональное антитело) |
l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)
l Synthesize one peptide (12-22aa, чистота>85%)
l Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l Immunize two New Zealand rabbits
l Titer the immunization by ELISA
l Post-bleed collection
l Antigen affinity purification |
л 2-3 мг пептидов/EA (нет- & диметилированный пептид)
l 1-2ml pre-bleed
l 2-5 мг антитела против неметилированного пептида
л 2-5 мг Лиза(Я2) специфическое антитело
l Project datasheet including ELISA and dot blot data |
13-16 недели |
| Поликлональный пакет 12 (Lys trimethylated specific polyclonal antibody) |
l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)
l Synthesize one peptide (12-22aa, чистота>85%)
l Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l Immunize two New Zealand rabbits
l Titer the immunization by ELISA
l Post-bleed collection
l Antigen affinity purification |
л 2-3 мг пептидов/EA (нет- & tri-methylated peptide)
l 1-2ml pre-bleed
l 2-5 мг антитела против неметилированного пептида
л 2-5 мг Лиза(Me3) специфическое антитело
l Project datasheet including ELISA and dot blot data |
13-16 недели |
| Polyclonal package13 (Arg methylation-specific polyclonal antibody) |
l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)
l Synthesize one peptide (12-22aa, чистота>85%)
l Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l Immunize two New Zealand rabbits
l Titer the immunization by ELISA
l Post-bleed collection
l Antigen affinity purification |
л 2-3 мг пептидов/EA (нет- & methylated peptide)
l 1-2ml pre-bleed
l 2-5 мг антитела против неметилированного пептида
l 2-5mg Arg(Мне) специфическое антитело
l Project datasheet including ELISA and dot blot data |
13-16 недели |
| Polyclonal package14 (Arg di-methylation (Symmetry)-specific polyclonal antibody) |
l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)
l Synthesize one peptide (12-22aa, чистота>85%)
l Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l Immunize two New Zealand rabbits
l Titer the immunization by ELISA
l Post-bleed collection
l Antigen affinity purification |
л 2-3 мг пептидов/EA (нет- & dimethylated peptide)
l 1-2ml pre-bleed
l 2-5 мг антитела против неметилированного пептида
l 2-5mg Arg(Я2) специфическое антитело
l Project datasheet including ELISA and dot blot data |
13-16 недели |
| Polyclonal package15 (Arg di-methylation (Asymmetric)-specific polyclonal antibody) |
l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)
l Synthesize one peptide (12-22aa, чистота>85%)
l Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l Immunize two New Zealand rabbits
l Titer the immunization by ELISA
l Post-bleed collection
l Antigen affinity purification |
л 2-3 мг пептидов/EA (нет- & dimethylated peptide)
l 1-2ml pre-bleed
l 2-5 мг антитела против неметилированного пептида
l 2-5mg Arg(Я2) специфическое антитело
l Project datasheet including ELISA and dot blot data |
13-16 недели |
| Поликлональный пакет 16 (Western guaranteed polyclonal antibody) |
l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)
l Synthesize one peptide (12-22aa, чистота>85%)
l Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l Immunize two New Zealand rabbits
l Titer the immunization by ELISA
l Post-bleed collection
l Antigen affinity purification
l Western blot confirmation |
l 2-3mg antigen peptide
l 1-2ml pre-bleed
l 2-5mg affinity purified antibody
l Project datasheet including ELISA and Western blot data |
15-18 недели |
| Поликлональный пакет17 (Western and IHC guaranteed polyclonal antibody) |
l Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision)
l Synthesize one peptide (12-22aa, чистота>85%)
l Conjugate the peptide to carrier protein (KLH, BSA or OVA)
l Immunize two New Zealand rabbits
l Titer the immunization by ELISA
l Post-bleed collection
l Antigen affinity purification
l WB and IHC confirmation |
l 2-3mg antigen peptide
l 1-2ml pre-bleed
l 2-5mg affinity purified antibody
l Project datasheet including ELISA, Western blot and IHC data |
15-18 недели |
