Sams Peptide CAS:125911-68-4

AMP-activatedproteinkinase plays a role in energy metabolism and glucose homeostasis by monitoring the ratio of ATP to AMP. This kinase can inactivate HMG-CoA reductase and acetyl-CoA carboxylase as well as block the activation of hormone-sensitive lipase by cyclic-AMP-dependent protein kinase (protein kinase A or PKA). SAMS peptide is a synthetic peptide substrate for AM/PK based on the sequence around Ser79 (which is phosphorylated exclusively by AM/PK) on acetyl-CoA carboxylase.1 In this sequence, Ser77 (which is phosphorylated exclusively by PKA) is replaced by alanine to eliminate a neighboring binding site for PKA.1 This substitution yields a substrate more specific for AM/PK than acetyl-CoA carboxylase itself. SAMS peptide is phosphorylated rapidly by AM/PK, providing a convenient and sensitive tool for assaying AM/PK activity.